Abstract

Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna. The species has evolved a multiple sex chromosome system with digametic males showing an X1YX2 karyotype and females an X1X1X2X2 karyotype. Fluorescence in situ hybridization, performed with a painting probe made from microdissected Y chromosomes, allowed a deeper insight on the chromosomal rearrangement, which underpinned the fusion event that generated the Y. Then, we used a DNA library established by microdissection and microcloning of the whole Y chromosome of Ch. hamatus for searching sex-linked sequences. One clone provided preliminary information on the presence on the Y chromosome of the CHD1 gene homologue, which is sex-linked in birds but in no other vertebrates. Several clones from the Y-chromosome mini-library contained microsatellites and transposable elements, one of which mapped to the q arm putative fusion region of the Y chromosome. The findings confirm that interspersed repetitive sequences might have fostered chromosome rearrangements and the emergence of the Y chromosome in Ch. hamatus. Detection of the CHD1 gene in the Y sex-determining region could be a classical example of convergent evolution in action.

Addeddate

2025-03-16 13:13:56

Bhl_virtual_titleid

210879

Bhl_virtual_volume

v.9:no.1 (2015)

Call number

10_3897_CompCytogen_v9i1_8731

Call-number

10_3897_CompCytogen_v9i1_8731

Foldoutcount

0

Genre

article

Identifier

lasermicrodisse9capr

Identifier-ark

ark:/13960/s2j26vkrj7r

Identifier-bib

10_3897_CompCytogen_v9i1_8731

Identifier-doi

10.3897/CompCytogen.v9i1.8731

Issue

1

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

0.9746

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

64

Page_number_module_version

1.0.5

Page_range

1-15

Pages

15

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Comparative Cytogenetics 9(1)

Year

2015

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